| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase ATR; Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1; ATR; FRP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATR recombinant protein (Position: E24-D1324). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects serine/threonine-protein kinase ATR in human samples and is validated for ICC/IF and ELISA. The predicted molecular weight is 301.4 kDa.
ATR is a serine/threonine kinase that responds to replication stress and single-stranded DNA. Recruited with its partner ATRIP to RPA-coated DNA, it phosphorylates CHK1 and other targets to slow the cell cycle, stabilize stalled replication forks and promote repair, and it is a major target in DNA damage response research.
| Target | Serine/threonine-protein kinase ATR (gene ATR; UniProt Q13535, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 301.4 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human ATR fragment (Glu24–Asp1324), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein kinase ATR in the nucleus, chromosome and nucleus envelope. Tissue expression noted by UniProt (human): Ubiquitous, with highest expression in testis...
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
