| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase ATR; Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1; ATR; FRP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATR recombinant protein (Position: E730-A1303). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects serine/threonine-protein kinase ATR in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 301.4 kDa, and the supplier reports an observed band at about 301 kDa.
ATR is a serine/threonine kinase that responds to replication stress and single-stranded DNA. Recruited with its partner ATRIP to RPA-coated DNA, it phosphorylates CHK1 and other targets to slow the cell cycle, stabilize stalled replication forks and promote repair, and it is a major target in DNA damage response research.
| Target | Serine/threonine-protein kinase ATR (gene ATR; UniProt Q13535, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 301.4 kDa |
| Observed MW | 301 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human ATR fragment (Glu730–Ala1303), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein kinase ATR in the nucleus, chromosome and nucleus envelope. Tissue expression noted by UniProt (human): Ubiquitous, with highest expression in testis... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jun Yan et al. (2026) Identification of a novel chalcone derivative as ferroptosis inducer through targeting TrxR in prostate cancer. Biochemical Pharmacology. 10.1016/j.bcp.2026.117760
