| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aurora kinase A;2.7.11.1;Aurora 2;Aurora/IPL1-related kinase 1;ARK-1;Aurora-related kinase 1;hARK1;Breast tumor-amplified kinase;Serine/threonine-protein kinase 15;Serine/threonine-protein kinase 6;Serine/threonine-protein kinase aurora-A;AURKA;AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Aurora A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DIH-1 is a rabbit monoclonal antibody that recognizes aurora kinase A (gene AURKA) in human and mouse samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 45.8 kDa, and the supplier reports an observed band at about 46 kDa.
Aurora kinase A (AURKA) is a mitotic serine/threonine kinase that localizes to centrosomes and spindle microtubules. Working with activators such as TPX2, it drives centrosome maturation, mitotic entry and bipolar spindle assembly. AURKA is often overexpressed in tumors and is a well-studied target of small-molecule kinase inhibitors.
| Target | Aurora kinase A (gene AURKA; UniProt O14965, human) |
|---|---|
| Clone | DIH-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.8 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human aurora A.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places aurora kinase A in the cytoplasm, cell projection and basolateral cell membrane. Tissue expression noted by UniProt (human): Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
