| Field | Specification |
|---|---|
| Target | |
| Alternative names | Axin-1; Axis inhibition protein 1; hAxin; AXIN1; AXIN |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AXIN1 recombinant protein (Position: M1-K811). Human AXIN1 shares 86.2% |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects axin-1 (gene AXIN1) in human samples and is validated for Western blot and ELISA.
Axin-1 (AXIN1) is the scaffold of the β-catenin destruction complex, bringing together APC, GSK3β and CK1 so that β-catenin is phosphorylated and degraded. It therefore acts as a key negative regulator of Wnt signaling, and loss-of-function mutations are found in several cancers, including hepatocellular carcinoma.
| Target | Axin-1 (gene AXIN1; UniProt O15169, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human AXIN1 fragment (Met1–Lys811), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places axin-1 in the cytoplasm, nucleus, membrane and cell membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
