| Field | Specification |
|---|---|
| Target | |
| Alternative names | Axin-2;Axin-like protein;Axil;Axis inhibition protein 2;Conductin;AXIN2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Axin2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAIA-1 is a rabbit monoclonal antibody that recognizes axin-2 (gene AXIN2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 93.6 kDa, and the supplier reports an observed band at about 94 kDa.
Axin-2 (AXIN2, also called conductin) is a scaffold protein of the β-catenin destruction complex and a negative regulator of Wnt signaling. Because AXIN2 is itself a direct Wnt target gene, its expression is widely used as a readout of Wnt/β-catenin pathway activity, and it marks Wnt-responsive stem cells in several tissues.
| Target | Axin-2 (gene AXIN2; UniProt Q9Y2T1, human) |
|---|---|
| Clone | AAIA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 93.6 kDa |
| Observed MW | 94 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): SW480 cells, PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human axin2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places axin-2 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in brain and lymphoblast. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ge Hongfei et al. (2021) Ambroxol Upregulates Glucocerebrosidase Expression to Promote Neural Stem Cells Differentiation Into Neurons Through Wnt/β-Catenin Pathway After Ischemic Stroke. Frontiers in Molecular Neuroscience. 10.3389/fnmol.2020.596039
