| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase receptor UFO (EC:2.7.10.1); AXL oncogene; AXL; UFO |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AXL recombinant protein (Position: A47-A244). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tyrosine-protein kinase receptor UFO (gene AXL) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 98.3 kDa, and the supplier reports an observed band at about 140 kDa.
AXL is a receptor tyrosine kinase of the TAM family (TYRO3, AXL, MER) that is activated by the ligand GAS6. It promotes cell survival, proliferation and migration and helps clear apoptotic cells and dampen innate immune responses. AXL overexpression is linked to epithelial–mesenchymal transition and drug resistance in cancer, making it an active research target.
| Target | Tyrosine-protein kinase receptor UFO (gene AXL; UniProt P30530, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 98.3 kDa |
| Observed MW | 140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human U2OS cells, human U-87MG cells, human PC-3 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human AXL fragment (Ala47–Ala244), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein kinase receptor UFO in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in metastatic colon tumors. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
