| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beta-secretase 1;3.4.23.46;Aspartyl protease 2;ASP2;Asp 2;Beta-site amyloid precursor protein cleaving enzyme 1;Beta-site APP cleaving enzyme 1;Memapsin-2;Membrane-associated aspartic protease 2;BACE1;BACE, KIAA1149; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BACE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABFF-2 is a rabbit monoclonal antibody that recognizes beta-secretase 1 (gene BACE1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 55.8 kDa, and the supplier reports an observed band at about 70 kDa.
β-secretase 1 (BACE1) is a membrane-bound aspartic protease that performs the first cleavage of the amyloid precursor protein (APP), which, followed by γ-secretase cleavage, generates amyloid-β peptides. Because of this role, BACE1 is a major subject of Alzheimer′s disease research; it also processes other substrates, including neuregulin-1.
| Target | Beta-secretase 1 (gene BACE1; UniProt P56817, human) |
|---|---|
| Clone | ABFF-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.8 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:40 |
Immunogen
Synthetic peptide based on the sequence of human BACE1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places beta-secretase 1 in the cell membrane, Golgi apparatus, endoplasmic reticulum and endosome. Tissue expression noted by UniProt (human): Expressed at high levels in the brain and pancreas.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wu Zhuoze et al. (2025) Pseudogene Lamr1-ps1 Aggravates Early Spatial Learning Memory Deficits in Alzheimer’s Disease Model Mice. Neuroscience Bulletin. 10.1007/s12264-024-01336-6
