| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beta-secretase 1;3.4.23.46;Aspartyl protease 2;ASP2;Asp 2;Beta-site amyloid precursor protein cleaving enzyme 1;Beta-site APP cleaving enzyme 1;Memapsin-2;Membrane-associated aspartic protease 2;BACE1;BACE, KIAA1149; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human BACE1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-BACE1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABFF-2; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-BACE1/Bace Rabbit Monoclonal Antibody catalog # M00322. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: BACE1 (Beta-secretase 1).
- Antibody format: Monoclonal; clone ABFF-2; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
BACE1 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. . Reported cellular localization context: Membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network . Endoplasmic reticulum. Endosome . Cell surface. Cytoplasmic vesicle membrane. Membrane raft . Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. Tissue expression notes (as provided): Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. .
Research relevance and current trends
- Research context keywords from the source record include: Alzheimer's Disease,Cell Biology,Neurodegenerative Disease,Neurology Process,Neuroscience,Proteolysis/Ubiquitin,Proteolytic Enzymes.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate BACE1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect BACE1 expression by Western blot in cell or tissue lysates, Enrich BACE1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 47 kDa; calculated MW: 55711 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 47 kDa
- Cellular localization (provided): Membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network . Endoplasmic reticulum. Endosome . Cell surface. Cytoplasmic vesicle membrane. Membrane raft . Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
- Tissue details (provided): Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
