| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fanconi anemia group J protein; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; DNA 5'-3' helicase FANCJ; BRIP1; BACH1; FANCJ |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BACH1/BRIP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23B65 is a rabbit monoclonal antibody that recognizes fanconi anemia group J protein (gene BRIP1) in human samples and is validated for Western blot. The predicted molecular weight is 140.9 kDa, and the supplier reports an observed band at about 110 kDa.
BRIP1 (also called FANCJ or BACH1) is a 5′–3′ DNA helicase that binds BRCA1 and acts in the Fanconi anemia pathway. It helps repair DNA interstrand crosslinks and double-strand breaks and resolves G-quadruplex structures during replication. BRIP1 mutations cause Fanconi anemia group J and increase ovarian cancer risk.
| Target | Fanconi anemia group J protein (gene BRIP1; UniProt Q9BX63, human) |
|---|---|
| Clone | 23B65 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 140.9 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BACH1/BRIP1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places fanconi anemia group J protein in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
