Anti-BAFF/TNFSF13B Antibody Picoband®

Overview

This antibody is intended for detection of TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-BAFF/TNFSF13B Antibody Picoband® catalog # PB9567. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human BAFF recombinant protein (Position: A134-L285). Human BAFF shares 86.1% amino acid (aa) sequence identity with mouse BAFF. (reported region: A134-L285).
• Molecular weight context: reported MW: 31 kDa; calculated MW: 31223 MW
• Reactivity: Human,Mouse,Rat
• Applications: WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 13B. BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas. The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34. The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa. Functional note: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. . Reported localization: Cell membrane; Single-pass type II membrane protein. Expression/tissue context: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. .

Research relevance and current trends

• Adaptive Immunity: Researchers commonly examine how TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) relates to this theme using model systems and orthogonal readouts.
• B Cells: Researchers commonly examine how TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) relates to this theme using model systems and orthogonal readouts.
• Cancer: Researchers commonly examine how TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) levels across conditions; band patterns may reflect isoforms and processing.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the tumor necrosis factor family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.