| Field | Specification |
|---|---|
| Target | |
| Alternative names | BAG family molecular chaperone regulator 2; BAG-2; Bcl-2-associated athanogene 2; BAG2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BAG2 Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEOE-2 is a rabbit monoclonal antibody that recognizes BAG family molecular chaperone regulator 2 (gene BAG2) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 23.8 kDa, and the supplier reports an observed band at about 24 kDa.
BAG2 is a co-chaperone of the HSP70/HSC70 system that acts as a nucleotide exchange factor to promote release of bound substrates. It can inhibit the ubiquitin ligase CHIP (STUB1), and it is studied in protein quality control and in the handling of misfolded proteins such as tau.
| Target | BAG family molecular chaperone regulator 2 (gene BAG2; UniProt O95816, human) |
|---|---|
| Clone | AEOE-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.8 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BAG2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
