| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BAG family molecular chaperone regulator 3;BAG-3;Bcl-2-associated athanogene 3;Bcl-2-binding protein Bis;Docking protein CAIR-1;BAG3;BIS; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Bag3 recombinant protein (Position: H100-N561). Human Bag3 shares 84.1% amino acid (aa) sequence identity with mouse Bag3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of BAG3 (BAG family molecular chaperone regulator 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Bag3 Antibody Picoband® catalog # PB9754. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Bag3 recombinant protein (Position: H100-N561). Human Bag3 shares 84.1% amino acid (aa) sequence identity with mouse Bag3. (reported region: H100-N561).
- Molecular weight context: reported MW: 80 kDa; calculated MW: 61595 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
BAG family molecular chaperone regulator 3; BAG family molecular chaperone regulator 3. BAG family molecular chaperone regulator 3 (BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones. BAG3 mutations are responsible for familial dilated cardiomyopathy. BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus. In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes. This process is called chaperone-assisted selective autophagy (CASA) and is essential for maintaining muscle activity in flies, mice and men. Functional note: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Reported localization: Cytoplasm, cytoskeleton . Endosome . Cytoplasmic vesicle, secretory vesicle, acrosome . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendrite . Cell projection, dendritic spine . Cell junction, synapse . Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane. . Expression/tissue context: Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .
Research relevance and current trends
- Apoptosis: Researchers commonly examine how BAG3 (BAG family molecular chaperone regulator 3) relates to this theme using model systems and orthogonal readouts.
- Apoptotic Markers: Researchers commonly examine how BAG3 (BAG family molecular chaperone regulator 3) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how BAG3 (BAG family molecular chaperone regulator 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative BAG3 (BAG family molecular chaperone regulator 3) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of BAG3 (BAG family molecular chaperone regulator 3) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
