| Field | Specification |
|---|---|
| Target | |
| Alternative names | Barrier-to-autointegration factor; Breakpoint cluster region protein 1; BANF1; BAF; BCRG1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BANF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20B35 is a rabbit monoclonal antibody that recognizes barrier-to-autointegration factor (gene BANF1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 10.1 kDa, and the supplier reports an observed band at about 10 kDa.
Barrier-to-autointegration factor (BANF1, BAF) is a small DNA-binding protein that bridges DNA strands and links chromatin to LEM-domain proteins of the nuclear envelope. It is essential for nuclear envelope reassembly after mitosis and chromatin organization, and it also restricts foreign DNA such as that of poxviruses. BANF1 mutations cause a progeroid syndrome.
| Target | Barrier-to-autointegration factor (gene BANF1; UniProt O75531, human) |
|---|---|
| Clone | 20B35 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.1 kDa |
| Observed MW | 10 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Immunogen
Synthetic peptide based on the sequence of human BANF1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places barrier-to-autointegration factor in the nucleus, chromosome, nucleus envelope and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
