| Field | Specification |
|---|---|
| Target | |
| Alternative names | BCAP29; BAP29; B-cell receptor-associated protein 29; BCR-associated protein 29; Bap29 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BAP29/BCAP29 recombinant protein (Position: L64-L241). Human BAP29/BCAP29 shares 79.2% amino acid (aa) sequence identity with mouse BAP29/BCAP29. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets B-cell receptor-associated protein 29 (gene BCAP29) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
BCAP29 is a multi-pass membrane protein of the endoplasmic reticulum closely related to BCAP31. It is thought to help export membrane proteins from the ER to the Golgi and may take part in caspase-8-dependent apoptosis, although its functions are less well defined than those of BCAP31.
| Target | B-cell receptor-associated protein 29 (gene BCAP29; UniProt Q9UHQ4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL (Human) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human BAP29/BCAP29 fragment (Leu64–Leu241), expressed in E. coli. Sequence identity with the mouse ortholog: 79.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places B-cell receptor-associated protein 29 in the endoplasmic reticulum membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
