| Field | Specification |
|---|---|
| Target | |
| Alternative names | Anti-Barzolvolimab Ab |
| UniProt # | |
| Species | |
| Reactivity | |
| Applications | |
| Assay type | |
| Sample type(s) | Serum | Plasma | Cell Culture Supernatant | Cell Lysate | Tissue Lysate | Other Liquid Samples |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The Anti-Barzolvolimab antibody ELISA Kit measures antibodies against Barzolvolimab in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double-antigen sandwich format: antigen pre-coated on the plate captures anti-Barzolvolimab antibody from the sample, and a biotin-labeled detection antigen then binds the captured antibody. HRP-Streptavidin and TMB substrate generate a color signal read at 450 nm, and concentration is calculated from the standard curve.
Specifications
| Alias | Anti-Barzolvolimab Ab ELISA Kit |
|---|---|
| Species | Universal |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Method | Sandwich ELISA, Double antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 1.563-100 ng/mL |
| Sensitivity | 0.938 ng/mL |
| Storage | 2-8°C (Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with Anti-Barzolvolimab Ab, no obvious cross reaction with other analogues. |
Kit Components
| Code | Component | Quantity |
|---|---|---|
| E001 | ELISA Microplate (dismountable) | 8 × 12 strips |
| E002 | Lyophilized Standard | 2 vials |
| E003 | Biotin-labeled Antigen (100X) | 120 µL |
| E034 | HRP-Streptavidin Conjugate (SABC, 100X) | 120 µL |
| E024 | TMB Substrate | 10 mL |
| E039 | Sample Dilution Buffer | 20 mL |
| E040 | Antigen Dilution Buffer | 10 mL |
| E049 | SABC Dilution Buffer | 10 mL |
| E026 | Stop Solution | 10 mL |
| E038 | Wash Buffer (25X) | 30 mL |
| E006 | Plate Sealer | 5 pieces |
| E007 | Product Description | 1 copy |
Performance Data
Recovery
| Serum | 90-102% (average 97%) |
|---|---|
| EDTA plasma | 88-105% (average 95%) |
| Heparin plasma | 87-102% (average 93%) |
Precision
| Intra-assay CV | 4.69-4.95% (3 samples, 3.03-49.17 ng/mL) |
|---|---|
| Inter-assay CV | 4.82-6.57% (3 samples, 3.1-50.48 ng/mL) |
Linearity (recovery after dilution)
| Serum | 1:2 86-105%; 1:4 82-100%; 1:8 80-95% |
|---|---|
| EDTA plasma | 1:2 85-105%; 1:4 83-95%; 1:8 80-89% |
| Heparin plasma | 1:2 90-104%; 1:4 85-95%; 1:8 85-95% |
Stability (sealed kit)
| 37 °C, 1 month | Average 80% |
|---|---|
| 2-8 °C, 6 months | 95-100% |
| 2-8 °C, 12 months | 85-98% |
Assay Procedure (Summary)
- Add 100 µL standard or sample; incubate 90 min at 37 °C.
- Wash 2 times; add 100 µL biotin-labeled antigen working solution; incubate 60 min at 37 °C.
- Wash 3 times; add 100 µL HRP-Streptavidin (SABC) working solution; incubate 30 min at 37 °C.
- Wash 5 times; add 90 µL TMB substrate; incubate 10-20 min at 37 °C.
- Add 50 µL stop solution and read at 450 nm immediately.
Required but not supplied: microplate reader (450 nm), 37 °C incubator (CO₂ incubator not recommended), plate washer or multichannel pipette, calibrated pipettes and tips, sterile tubes, absorbent paper, and deionized or distilled water.
Sample Notes
- Serum and plasma should be diluted at least 1:2 with the sample dilution buffer.
- Maximum volume per well: serum, plasma or other liquids 50 µL; cell culture supernatant or cell/tissue lysate 100 µL.
- Store samples below 5 days at 2-8 °C, up to 6 months at -20 °C, or up to 2 years at -80 °C.
- Avoid hemolyzed or lipemic samples, and avoid NP-40, Triton X-100 or DTT in lysis buffers.
Storage & Handling
For Research Use Only. Not for use in diagnostic procedures.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
