| Field | Specification |
|---|---|
| Target | |
| Alternative names | Large proline-rich protein BAG6; BAG family molecular chaperone regulator 6; BCL2-associated athanogene 6; BAG-6; HLA-B-associated transcript 3; Protein G3; Protein Scythe; BAG6; BAT3; G3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BAT3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25B57 is a rabbit monoclonal antibody that recognizes large proline-rich protein BAG6 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 119.4 kDa, and the supplier reports an observed band at about 150 kDa.
BAG6 (also called BAT3) is an ATP-independent chaperone that recognizes exposed hydrophobic segments on misfolded or mislocalized proteins. As part of the BAG6 complex it hands tail-anchored membrane proteins to the insertion pathway or routes defective proteins to ubiquitination and proteasomal degradation, and it has additional roles in immune regulation.
| Target | Large proline-rich protein BAG6 (gene BAG6; UniProt P46379, human) |
|---|---|
| Clone | 25B57 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 119.4 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BAT3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes large proline-rich protein BAG6 as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed by immature dendritic cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
