Anti-Bax

SKU:BHA10700473
Overview
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Bax-specific rabbit monoclonal antibody. FITC-conjugated. IHC-P validated at See datasheet in Human samples. In vitro cloning technology ensures high specificity.
Conjugate FITC
Application IHC-P Optimized
Technology In Vitro Cloned
Clone T22-A
Options selector
Catalog no. Size
DB 1010 100 tests
Available Options
Size
Field Specification
Mfr No DB 1010
Clonality
  • Monoclonal
Formulation Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
Host Rabbit
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
Storage Store undiluted at 4°C, protect from prolonged exposure to light. Do not freeze.
Target Bax

Product Overview

IHC Primary Antibody

IHC-optimized FITC-conjugated; antibody targeting Bax. Designed for paraffin-embedded tissue sections (IHC-P). Validated for use at 1:100 dilution.

Validated Application

Application Recommended Dilution
Immunohistochemistry – Paraffin (IHC-P) See datasheet
✔ IHC-Optimized | DB Biotech Inc

Antibody Specifications

Target Bax
Host Rabbit
Clonality Monoclonal
Isotype IgG
Conjugate FITC
Reactivity Human
Purification Affinity Purification
Immunogen nan
Clone T22-A
Formulation Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
Storage Buffer nan
Storage Store undiluted at 4°C, protect from prolonged exposure to light. Do not freeze. Protect from light.

Production & Preparation

The antibody was prepared by in vitro cloning technology, and the monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Contains sodium azide. Light-sensitive — protect from prolonged light exposure.
TargetBax
HostRabbit
ClonalityMonoclonal
ConjugateFITC
ReactivityHuman
ApplicationIHC-P
DilutionSee datasheet
PurificationAffinity Purification
CloneT22-A
Immunogennan

Validated for IHC-P on paraffin-embedded sections.

ApplicationDilution
IHC-P (Paraffin)See datasheet

Immunogen: nan

Clone: T22-A

What tissue types has this antibody been optimized for?
This antibody has been optimized for immunohistochemistry on paraffin-embedded (IHC-P) tissue sections from Human samples. Performance in frozen sections or other formats may require additional optimization.
What is the recommended working dilution?
The validated starting dilution is See datasheet for IHC-P. Optimal dilution may vary depending on tissue fixation, antigen retrieval method, and detection system. Titration is recommended for each new tissue type.
How was this antibody produced?
This is a rabbit monoclonal antibody produced by in vitro cloning technology. The clone designation is T22-A. This production method ensures a monospecific product with consistent lot-to-lot performance — critical for reproducible IHC results across experiments.
What antigen retrieval method is recommended?
Heat-induced epitope retrieval (HIER) in citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) is generally recommended for paraffin-embedded sections. The optimal retrieval method may vary by antibody and tissue type.
What storage conditions are required?
Store undiluted at 4°C. Do not freeze. Protect from prolonged exposure to light — FITC is light-sensitive.
Is a secondary antibody required with this product?
No. This antibody is directly FITC-conjugated and can be detected without a secondary antibody. This simplifies the IHC workflow and reduces non-specific background from secondary antibody cross-reactivity.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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