| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 13;Angie;B cell-attracting chemokine 1;BCA-1;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;CXCL13;BCA1, BLC, SCYB13; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human BCA1 recombinant protein (Position: V23-P109). Human BCA1 shares 45.3% amino acid (aa) sequence identity with mouse BCA1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CXCL13 (C-X-C motif chemokine 13) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-BCA1/CXCL13 Antibody Picoband® catalog # PB9697. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human BCA1 recombinant protein (Position: V23-P109). Human BCA1 shares 45.3% amino acid (aa) sequence identity with mouse BCA1. (reported region: V23-P109).
- Molecular weight context: reported MW: 13 kDa; calculated MW: 12664 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-X-C motif chemokine 13; C-X-C motif chemokine 13. C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells. Functional note: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Reported localization: Secreted. Expression/tissue context: Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how CXCL13 (C-X-C motif chemokine 13) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how CXCL13 (C-X-C motif chemokine 13) relates to this theme using model systems and orthogonal readouts.
- Chemokines: Researchers commonly examine how CXCL13 (C-X-C motif chemokine 13) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CXCL13 (C-X-C motif chemokine 13) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
