| Field | Specification |
|---|---|
| Target | |
| Alternative names | Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Placental protein 18; PP18; BCAT2; BCATM; BCT2; ECA40 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BCAT2 recombinant protein (Position: A11-E363). Human BCAT2 shares 83.6% and 81.9% amino acid (aa) sequence identity with mouse and rat BCAT2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets branched-chain-amino-acid aminotransferase, mitochondrial (gene BCAT2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 44.3 kDa, and the supplier reports an observed band at about 39 kDa.
BCAT2 is the mitochondrial branched-chain aminotransferase that catalyzes the first step in the breakdown of leucine, isoleucine and valine in most peripheral tissues, notably skeletal muscle. It feeds branched-chain keto acids to the BCKD complex and is studied in metabolic health, insulin resistance and adipocyte lipid synthesis.
| Target | Branched-chain-amino-acid aminotransferase, mitochondrial (gene BCAT2; UniProt O15382, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 44.3 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells, human 293T cells, human A431 cells, rat kidney tissue, rat heart tissue, rat stomach tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon adenocarcinoma tissue, human lung cancer tissue, human urothelial carcinoma tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human BCAT2 fragment (Ala11–Glu363), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 83.6% and 81.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places branched-chain-amino-acid aminotransferase, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
