| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase; BCHE; CHE1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BCHE recombinant protein (Position: E29-L602). Human BCHE shares 80.8% amino acid (aa) sequence identity with mouse BCHE. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cholinesterase (gene BCHE) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 68.4 kDa, and the supplier reports an observed band at about 85–90 kDa.
Butyrylcholinesterase (BCHE) is a serine esterase made in the liver and found in plasma, with broader substrate specificity than acetylcholinesterase. It hydrolyzes acetylcholine and choline esters as well as drugs such as succinylcholine, and it scavenges organophosphate nerve agents and pesticides. Genetic variants that lower its activity cause prolonged responses to certain muscle relaxants.
| Target | Cholinesterase (gene BCHE; UniProt P06276, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 68.4 kDa |
| Observed MW | 85–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human BCHE fragment (Glu29–Leu602), expressed in E. coli. Sequence identity with the mouse ortholog: 80.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes cholinesterase as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
