| Field | Specification |
|---|---|
| Target | |
| Alternative names | Branched-chain alpha-ketoacid dehydrogenase kinase; BCKDH kinase; BCKDHKIN; BDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; BCKDK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BCKDK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29B01 is a rabbit monoclonal antibody that recognizes branched-chain alpha-ketoacid dehydrogenase kinase (gene BCKDK) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 46.4 kDa, and the supplier reports an observed band at about 46 kDa.
Branched-chain α-ketoacid dehydrogenase kinase (BCKDK) is a mitochondrial kinase that phosphorylates and inactivates the BCKD complex, thereby slowing branched-chain amino acid breakdown. It works as a regulatory pair with the phosphatase PPM1K, and it is studied in metabolic diseases, insulin resistance and neurodevelopment.
| Target | Branched-chain alpha-ketoacid dehydrogenase kinase (gene BCKDK; UniProt O14874, human) |
|---|---|
| Clone | 29B01 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.4 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BCKDK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places branched-chain alpha-ketoacid dehydrogenase kinase in the mitochondrion matrix. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
