| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptosis regulator Bcl-2;BCL2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Bcl-2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAOH-2 is a rabbit monoclonal antibody that recognizes apoptosis regulator Bcl-2 (gene BCL2) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 26.3 kDa, and the supplier reports an observed band at about 26 kDa.
Bcl-2 (BCL2) is an anti-apoptotic protein located mainly on the mitochondrial outer membrane that binds and restrains pro-apoptotic Bcl-2 family members such as BAX, BAK and BH3-only proteins. It was first identified through the t(14;18) translocation in follicular lymphoma, and it is a central regulator of mitochondrial apoptosis and a target of BH3-mimetic drugs.
| Target | Apoptosis regulator Bcl-2 (gene BCL2; UniProt P10415, human) |
|---|---|
| Clone | AAOH-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.3 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung adenocarcinoma tissue, human thyroid cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human bcl-2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places apoptosis regulator Bcl-2 in the mitochondrion outer membrane, nuclear membrane, endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao-Fei Lv et al. (2020) TMEM16A ameliorates vascular remodeling by suppressing autophagy via inhibiting Bcl-2-p62 complex formation. Theranostics. 10.7150/thno.41028
Longlong Li et al. (2021) Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate. Oxidative Medicine and Cellular Longevity. 10.1155/2021/5594059
Yi Lingxian et al. (2024) Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion. Molecular Biomedicine. 10.1186/s43556-024-00203-0
Yanbing Wang et al. (2022) TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis. Biology-Basel. 10.3390/biology11010141
Xiaodong Zhang et al. (2017) Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling. Oncotarget. 10.18632/oncotarget.14523
Ranran Hou et al. (2020) Polysaccharide from Echinacea purpurea reduce the oxidant stress in vitro and in vivo. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2020.01.129
Zhang Zijuan et al. (2022) Neuroprotective Effects of a Cholecystokinin Analogue in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine Parkinson’s Disease Mouse Model. Frontiers in Neuroscience. 10.3389/fnins.2022.814430
Yingran Liang et al. (2020) Crocin ameliorates arsenic trioxide‑induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2020.110713
Jing-Jing Xing et al. (2019) Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice. Antioxidants. 10.3390/antiox8090347
Kejing Li et al. (2023) Induction of apoptosis and autophagy by dichloromethane extract from Patrinia scabiosaefolia Fisch on acute myeloid leukemia cells. Environmental Toxicology. 10.1002/tox.24090
