| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-like protein 1;Bcl2-L-1;Apoptosis regulator Bcl-X;BCL2L1;BCL2L, BCLX; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Bcl-XS, different from the related rat and mouse sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects bcl-2-like protein 1 (gene BCL2L1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 26.0 kDa, and the supplier reports an observed band at about 18–20 kDa.
Bcl-xL (BCL2L1) is an anti-apoptotic Bcl-2 family protein that binds pro-apoptotic partners such as BAX, BAK and BH3-only proteins to prevent mitochondrial outer membrane permeabilization. Alternative splicing produces the pro-apoptotic Bcl-xS isoform. Bcl-xL is important for survival of many cell types, including platelets and neurons, and is a target of BH3-mimetic drugs.
| Target | Bcl-2-like protein 1 (gene BCL2L1; UniProt Q07817, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 26.0 kDa |
| Observed MW | 18–20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human U87 cells, rat pancrease tissue, mouse pancrease tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human bcl-XS. It differs from the rat and mouse sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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