| Field | Specification |
|---|---|
| Target | |
| Alternative names | B-cell lymphoma 3 protein; BCL-3; Proto-oncogene BCL3; BCL3; BCL4; D19S37 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanBCL3recombinantprotein(Position:E118-A366).HumanBCL3?shares?86.7%?amino?acid?(aa)?sequence?identity?withmouseBCL3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets B-cell lymphoma 3 protein (gene BCL3) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 47.6 kDa, and the supplier reports an observed band at about 60 kDa.
BCL3 is an IκB family protein with ankyrin repeats that binds the NF-κB p50 and p52 homodimers. Depending on context, it can act as a transcriptional coactivator or corepressor of NF-κB target genes, and it was first identified at a chromosomal translocation in B-cell chronic lymphocytic leukemia.
| Target | B-cell lymphoma 3 protein (gene BCL3; UniProt P20749, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 47.6 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
E. coli-derivedhumanBCL3recombinantprotein(Position:E118-A366).HumanBCL3?shares?86.7%?amino?acid?(aa)?sequence?identity?withmouseBCL3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places B-cell lymphoma 3 protein in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hao Yin et al. (2025) METTL3-YTHDF1 axis drives BCL-3 m6A methylation to promote the ferroptosis of brain microvascular endothelial cells during intracerebral hemorrhage. Brain Research Bulletin. 10.1016/j.brainresbull.2025.111434
