| Field | Specification |
|---|---|
| Target | |
| Alternative names | Brain-derived neurotrophic factor;BDNF;Bdnf; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Anti-BDNF Picoband™ Antibody (PB9075) was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets neurotrophic factor BDNF precursor form in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 28.1 kDa, and the supplier reports an observed band at about 14 kDa.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted as proBDNF and processed to mature BDNF, which signals mainly through the TrkB receptor (NTRK2). It supports survival and differentiation of neurons during development and regulates synaptic plasticity, learning and memory in the adult brain, making it a common target in neuroscience and psychiatry research.
| Target | Neurotrophic factor BDNF precursor form (gene Bdnf; UniProt P21237, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.1 kDa |
| Observed MW | 14 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): recombinant human BDNF protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Anti-BDNF Picoband™ Antibody (PB9075) was raised against E. coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes neurotrophic factor BDNF precursor form as a secreted protein. Tissue expression noted by UniProt (mouse): Expressed in the dorsal root ganglion and the spinal cord (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Ting et al. (2022) SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells. Journal of Nanobiotechnology. 10.1186/s12951-022-01337-5
Yuan-Huan Ma et al. (2018) Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord. Biomaterials. 10.1016/j.biomaterials.2018.01.015
Junhan Cao et al. (2022) Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2022.11.243
Ruike Wang et al. (2022) SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2022.07.060
Huimao Liu et al. (2026) Resveratrol Attenuates Neuroinflammation and Gut–Brain Axis Dysfunction Induced by Lead and Cadmium Co-Exposure by Modulating Gut Microbiota in Rats. Phytotherapy Research. 10.1002/ptr.70301
Li Mo et al. (2024) Human induced pluripotent stem cell/embryonic stem cell-derived pyramidal neuronal precursors show safety and efficacy in a rat spinal cord injury model. Cellular and Molecular Life Sciences. 10.1007/s00018-024-05350-9
Li XiaoBo et al. (2018) Intravenously Delivered Allogeneic Mesenchymal Stem Cells Bidirectionally Regulate Inflammation and Induce Neurotrophic Effects in Distal Middle Cerebral Artery Occlusion Rats Within the First 7 Days After Stroke. Cellular Physiology and Biochemistry. 10.1159/000489384
Tong Chuanwang et al. (2022) Study on the mechanism of visual aging in cats’ primary visual cortex based on BDNF-TrkB signal pathway. Scientific Reports. 10.1038/s41598-022-14918-z
Marina Estrázulas et al. (2021) Central and peripheral effects of environmental enrichment in a mouse model of arthritis. International Immunopharmacology. 10.1016/j.intimp.2021.108386
Junjie Yu et al. (2025) Construction of ROS-responsive cascade-targeted liposomes and investigation of their anti-AD effects. International Immunopharmacology. 10.1016/j.intimp.2025.115234
