| Field | Specification |
|---|---|
| Target | |
| Alternative names | Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BDNF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BEH-2 is a rabbit monoclonal antibody that recognizes neurotrophic factor BDNF precursor form in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 27.8 kDa, and the supplier reports an observed band at about 28 kDa.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted as proBDNF and processed to mature BDNF, which signals mainly through the TrkB receptor (NTRK2). It supports survival and differentiation of neurons during development and regulates synaptic plasticity, learning and memory in the adult brain, making it a common target in neuroscience and psychiatry research.
| Target | Neurotrophic factor BDNF precursor form (gene BDNF; UniProt P23560, human) |
|---|---|
| Clone | BEH-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.8 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human esophageal carcinoma tissue, human lung tissue, rat stomach tissue, mouse heart tissue, rat pancreas tissue, human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human BDNF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes neurotrophic factor BDNF precursor form as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma and in saliva (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haoyuan Li et al. (2023) Engineering brain-derived neurotrophic factor mRNA delivery for the treatment of Alzheimer’s disease. Chemical Engineering Journal. 10.1016/j.cej.2023.143152
Chang Rui et al. (2023) The hippocampal FTO-BDNF-TrkB pathway is required for novel object recognition memory reconsolidation in mice. Translational Psychiatry. 10.1038/s41398-023-02647-4
Li Mo et al. (2024) Human induced pluripotent stem cell/embryonic stem cell-derived pyramidal neuronal precursors show safety and efficacy in a rat spinal cord injury model. Cellular and Molecular Life Sciences. 10.1007/s00018-024-05350-9
Yan Wang et al. (2025) Revealing the effective components and mechanism of Zhimu-Huangbai herb-pair in the treatment of Alzheimer's disease. Journal of Ethnopharmacology. 10.1016/j.jep.2025.119699
Guoyu Zhou et al. (2021) Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.1c04907
Yajun Qiao et al. (2025) Identification and Validation of Antidepressant Small Molecules Using Bioinformatics and Mouse Depression Models. Drug Design Development and Therapy. 10.2147/DDDT.S537918
Wen Yuxin et al. (2024) Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance. Molecular Neurobiology. 10.1007/s12035-023-03860-4
Haiwang Song et al. (2025) Low-Frequency (1 Hz) Repetitive Trans-Spinal Magnetic Stimulation Attenuates NLRP3-Driven Neuroinflammation and Enhances Motor Recovery in Spinal Cord-Injured Mice. Journal of Inflammation Research. 10.2147/JIR.S516006
Wan-ying Liu et al. (2024) Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer’s Disease. ACS Chemical Neuroscience. 10.1021/acschemneuro.4c00109
Sining Feng et al. (2021) Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats. Molecular Medicine Reports. 10.3892/mmr.2021.11934
