| Field | Specification |
|---|---|
| Target | |
| Alternative names | Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BDNF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17B21 is a rabbit monoclonal antibody that recognizes neurotrophic factor BDNF precursor form in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 27.8 kDa, and the supplier reports an observed band at about 28 kDa.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted as proBDNF and processed to mature BDNF, which signals mainly through the TrkB receptor (NTRK2). It supports survival and differentiation of neurons during development and regulates synaptic plasticity, learning and memory in the adult brain, making it a common target in neuroscience and psychiatry research.
| Target | Neurotrophic factor BDNF precursor form (gene BDNF; UniProt P23560, human) |
|---|---|
| Clone | 17B21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.8 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human breast cancer tissue, human Hodgkin's lymphoma tissue, human prostate cancer tissue, human brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human BDNF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes neurotrophic factor BDNF precursor form as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma and in saliva (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
