| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tyrosinase;1.14.18.1;LB24-AB;Monophenol monooxygenase;SK29-AB;Tumor rejection antigen AB;TYR; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Beclin 1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-BECN1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADA-2; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-Beclin 1 BECN1 Monoclonal Antibody catalog # M00327. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: BECN1 (Tyrosinase).
- Antibody format: Monoclonal; clone ADA-2; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
BECN1 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Reported cellular localization context: Melanosome membrane ; Single-pass type I membrane protein . Tissue expression notes (as provided): Widely expressed; lower levels in brain and lung.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Susceptibility,Cell Death,Epigenetics and Nuclear Signaling,Interspecies Interaction,Metabolism,Metabolism Processes,Microbiology,Oncoproteins/Suppressors,Pathways and Processes,Signal Transduction,Transcription,Tumor Suppressors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate BECN1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect BECN1 expression by Western blot in cell or tissue lysates, Detect BECN1 in FFPE tissue sections by immunohistochemistry, Localize BECN1 by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 47 kDa; calculated MW: 60393 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 47 kDa
- Cellular localization (provided): Melanosome membrane ; Single-pass type I membrane protein .
- Tissue details (provided): Widely expressed; lower levels in brain and lung.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
