| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Beclin 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADA-2 is a rabbit monoclonal antibody that recognizes beclin-1 (gene BECN1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 51.9 kDa, and the supplier reports an observed band at about 55 kDa.
Beclin-1 (BECN1) is a core subunit of the class III phosphatidylinositol 3-kinase (VPS34) complex, which generates phosphatidylinositol 3-phosphate to start autophagosome formation. It also functions in endocytic trafficking, and its activity is controlled by binding partners such as Bcl-2. Beclin-1 is a widely used marker in autophagy research.
| Target | Beclin-1 (gene BECN1; UniProt Q14457, human) |
|---|---|
| Clone | ADA-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.9 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HEK293 cells, NIH/3T3 cells, C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human beclin 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places beclin-1 in the cytoplasm, endosome membrane, endoplasmic reticulum membrane and mitochondrion membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhiqiang Feng et al. (2024) Liensinine sensitizes colorectal cancer cells to oxaliplatin by targeting HIF-1a to inhibit autophagy. Phytomedicine. 10.1016/j.phymed.2024.155647
Chen Feng et al. (2024) Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability. Cardiovascular Toxicology. 10.1007/s12012-024-09865-2
Mao Miaobin et al. (2025) Collagen type I alpha 1 induces radio-resistance in hypoxic glioblastoma cells by promoting autophagy. Journal of Neuro-Oncology. 10.1007/s11060-025-05132-8
Jialin Zhu et al. (2018) Beclin1 overexpression suppresses tumor cell proliferation and survival via an autophagy‑dependent pathway in human synovial sarcoma cells. Oncology Reports. 10.3892/or.2018.6599
