Anti-beta Casein Rabbit Monoclonal Antibody

SKU:BHA21009364
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-CSN2 antibody (Rabbit monoclonal, clone 18C65, IgG). Validated for Western blot and immunoprecipitation. Reactivity: Human. Recognizes beta-casein. Observed band in Western blot: about 25 kDa.
Target CSN2
clone number 18C65
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, IP
Options selector
Catalog no. Size Conjugation
M03701 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IP
      Application details: WB 1:500-2000<br>IP 1:50<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target CSN2
Alternative names Beta-casein; CSN2; CASB
Gene ID 1447
UniProt # P05814
Host Rabbit
Clone 18C65
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human beta Casein
Molecular weight 25.4 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M03701
Main SKU BHA21009364
Rabbit Monoclonal

Product Overview

Clone 18C65 is a rabbit monoclonal antibody that recognizes beta-casein (gene CSN2) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 25.4 kDa, and the supplier reports an observed band at about 25 kDa.

Beta-casein (CSN2) is one of the main milk proteins made by mammary epithelial cells. Together with other caseins it forms casein micelles and helps define their surface properties, supporting the delivery of calcium and amino acids to the newborn. It is a standard marker of mammary differentiation and lactation.

Target Beta-casein (gene CSN2; UniProt P05814, human)
Clone 18C65
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 25.4 kDa
Observed MW 25 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunoprecipitation 1:50

Samples with a confirmed band (WB): human milk.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human beta Casein.

Reactivity Notes

The supplier lists reactivity with human. UniProt describes beta-casein as a secreted protein. Tissue expression noted by UniProt (human): Mammary gland specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 25.4 kDa; observed band at about 25 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human beta Casein.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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