| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beta-glucuronidase; Gusb; Gus, Gus-s |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse beta glucuronidase/Gusb recombinant protein (Position: R122-E575). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-beta glucuronidase/Gusb Antibody Picoband® is an antibody for Gusb detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Gusb (glucuronidase, beta); UniProt: P12265
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 75 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-beta glucuronidase/Gusb Antibody Picoband® catalog # A02234-2.
Biological background
Biological context: Plays an important role in the degradation of dermatan and keratan sulfates.
Common research applications
- Western blotting (WB): Compare Gusb levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Gusb in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Gusb-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.
- Cross reactivity: No cross-reactivity with other proteins.
- Research category: Auditory System,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Neurogenesis,Neurology Process,Neuroscience,Nuclear,Sensory System,Signaling Pathways,Stem Cells,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
