Anti-Beta-nerve growth factor NGF Antibody Picoband®

SKU:BHA21006599
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-NGF antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot and ELISA. Reactivity: Human. Recognizes beta-nerve growth factor. Observed band in Western blot: about 22 kDa.
Target NGF
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
RP1025 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated; 100 ug/vial — Carrier Free
      Contents: Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify Carrier Free or No BSA in your order note.
      Form: Lyophilized
      Applications: ELISA,WB
      Application details: Western blot, 0.1-0.5μg/ml, Human, -
      ELISA, 0.1-0.5μg/ml, -
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial — Biotin; 100 ug/vial — Cy3; 100 ug/vial — Fluoro488; 100 ug/vial — Fluoro550; 100 ug/vial — Fluoro594; 100 ug/vial — FITC; 100 ug/vial — APC; 100 ug/vial — PE; 100 ug/vial — Fluoro647
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      Form: Liquid
      Applications: Flow Cytometry | WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial — HRP
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
      Form: Liquid
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NGF
Alternative names Beta-nerve growth factor;Beta-NGF;NGF;NGFB;
Gene ID 4803
UniProt # P01138
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • ELISA
Immunogen E. coli-derived human NGFB recombinant protein (Position: S122-A241).
Molecular weight 27.0 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) RP1025
Main SKU BHA21006599
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody recognizes beta-nerve growth factor (gene NGF) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 27.0 kDa, and the supplier reports an observed band at about 22 kDa.

Nerve growth factor (NGF) is the founding member of the neurotrophin family. Acting through its high-affinity receptor TrkA (NTRK1) and p75NTR (NGFR), it supports survival and growth of sympathetic and sensory neurons, while its precursor proNGF can signal cell death through p75NTR and sortilin family co-receptors; NGF is also a major mediator of pain.

Target Beta-nerve growth factor (gene NGF; UniProt P01138, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 27.0 kDa
Observed MW 22 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.1–0.5 µg/mL (Human)
ELISA 0.1–0.5 µg/mL

Immunogen

Recombinant human NGFB fragment (Ser122–Ala241), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with human. UniProt describes beta-nerve growth factor as secreted and also located in the endosome lumen. A carrier-free (BSA-free) format can be requested; specify it in the order note.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Human); ELISA 0.1–0.5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Is a blocking reagent available for this antibody?
A.A matching blocking reagent for competition (specificity) controls can be supplied on request. Contact info@biohippo.com to request it.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 27.0 kDa; observed band at about 22 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Recombinant human NGFB fragment (Ser122–Ala241), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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