Anti-BEX3 Antibody Picoband®

Overview

Anti-BEX3 Antibody Picoband® is an antibody for BEX3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: BEX3 (one cut homeobox 2); UniProt: Q00994
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 28 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-BEX3 Antibody Picoband® catalog # A09675-2.

Biological background

Biological context: Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.

Expression and localization notes: cellular localization: Nucleus., tissue context: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines..

Common research applications

• Western blotting (WB): Compare BEX3 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of BEX3 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify BEX3-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Protein BEX3 is a protein that in humans is encoded by the NGFRAP1 gene. NGFRAP1 (nerve growth factor receptor associated protein 1), also called BEX3 (brain-expressed X-linked protein 3), NADE (p75NTR-associated death executor) or FAP-1 (Fas-associated phosphatase-1), is a 24 kDa member of the BEX protein family. A nuclear export signal (aa 77-87) allows NGFRAP1 to export bound p75NTR. It is widely expressed, but highest in ovarian granulosa cells, testis, prostate, seminal vesicle and liver. Except for a 13-19 amino acid (aa) rodent Arg/His region at aa 35 that is not found in humans, human NGFRAP1 shares 94% aa identity with mouse and rat NGFRAP1. In humans, a 101 aa isoform with an alternative start site at aa 11 has been described.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus.
• Tissue details: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
• Research category: Epigenetics and Nuclear Signaling,Transcription,Transcription Factors

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.