| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bcl-2-interacting killer;Apoptosis inducer NBK;BIP1;BP4;BIK;NBK; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with mouse Bik. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of BIK (Bcl-2-interacting killer) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Bik Antibody Picoband® catalog # PB9755. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with mouse Bik. (reported region: M1-R123).
- Molecular weight context: reported MW: 22 kDa; calculated MW: 18016 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Bcl-2-interacting killer; Bcl-2-interacting killer. Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. Functional note: Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X (L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. . Reported localization: Endomembrane system; Single-pass membrane protein. Mitochondrion membrane ; Single-pass membrane protein . Around the nuclear envelope, and in cytoplasmic membranes. Expression/tissue context: Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .
Research relevance and current trends
- Apoptosis: Researchers commonly examine how BIK (Bcl-2-interacting killer) relates to this theme using model systems and orthogonal readouts.
- Associated Proteins: Researchers commonly examine how BIK (Bcl-2-interacting killer) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how BIK (Bcl-2-interacting killer) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative BIK (Bcl-2-interacting killer) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of BIK (Bcl-2-interacting killer) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
