| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-interacting killer;Apoptosis inducer NBK;BIP1;BP4;BIK;NBK; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with mouse Bik. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets bcl-2-interacting killer (gene BIK) in human, mouse and rat samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 18.0 kDa, and the supplier reports an observed band at about 22 kDa.
BIK (Bcl-2-interacting killer) is a BH3-only pro-apoptotic Bcl-2 family protein located mainly in the endoplasmic reticulum. It binds anti-apoptotic proteins such as Bcl-2 and Bcl-xL and promotes apoptosis, including through release of ER calcium and effects on mitochondria.
| Target | Bcl-2-interacting killer (gene BIK; UniProt Q13323, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 18.0 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat spleen tissue, rat gaster tissue, rat intestine tissue, MCF-7 cells, A549 cells, SKOV cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue, human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): MCF-7 cells, THP-1 cells, A431 cells.
Immunogen
Recombinant human Bik fragment (Met1–Arg123), expressed in E. coli. Sequence identity with the mouse ortholog: 42.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places bcl-2-interacting killer in the endomembrane system and mitochondrion membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
