| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bcl-2-like protein 11;Bcl2-L-11;Bcl2-interacting mediator of cell death;BCL2L11;BIM; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Bim |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Bim BCL2L11 Rabbit Monoclonal Antibody is an antibody targeting BCL2L11. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CBE-2; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 65-80 kDa; calculated MW: 22171 MW.
Boster Bio Anti-Bim BCL2L11 Rabbit Monoclonal Antibody catalog # M01552. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: BCL2L11 — Bcl-2-like protein 11
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CBE-2; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 65-80 kDa; Calculated: 22171 MW
Biological background
Protein function (datasheet): Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. .
Cellular localization (datasheet): Endomembrane system ; Peripheral membrane protein . Associated with intracytoplasmic membranes. .
Tissue details (datasheet): Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. .
Research relevance and current trends
- Commonly studied in contexts related to Alzheimer's Disease,Apoptosis,Apoptotic Markers,Associated Proteins,Cancer,Cell Biology,Cell Death,Immunology,Innate Immunity,Intracellular,Invasion/Microenvironment,Macrophage/Inflammation,MAPK Pathway,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
