| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1; BIN1; AMPHL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BIN1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30B93 is a rabbit monoclonal antibody that recognizes myc box-dependent-interacting protein 1 (gene BIN1) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 64.7 kDa; the supplier reports observed bands at 50, 60, 65 kDa.
BIN1 (also called amphiphysin 2) is a BAR-domain protein that senses and induces membrane curvature. Muscle isoforms are needed to form T-tubules, and brain isoforms act in endocytosis and synaptic function. BIN1 mutations cause centronuclear myopathy, and common variants near BIN1 are among the strongest genetic risk factors for late-onset Alzheimer′s disease.
| Target | Myc box-dependent-interacting protein 1 (gene BIN1; UniProt O00499, human) |
|---|---|
| Clone | 30B93 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 64.7 kDa |
| Observed MW | 50, 60, 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BIN1.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
