| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Proline-rich AKT1 substrate 1;40 kDa proline-rich AKT substrate;AKT1S1 ;PRAS40 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human BLNK |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Boster Bio Anti-BLNK Rabbit Monoclonal Antibody catalog # M03630-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Protein: T-cell surface glycoprotein CD3 zeta chain | Gene: Proline-rich AKT1 substrate 1 | Target: BLNK | Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. . | Research areas: Cell Biology,Cell Cycle,Intracellular Signaling,Kinases,Kinases/Phosphatases,Neuroscience,Neurotransmission,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction
Host: Rabbit | Clonality/Clone: Monoclonal / Clone: 28B74 | Isotype: IgG | Reactivity: Human,Mouse,Rat | Immunogen: A synthesized peptide derived from human BLNK | Concentration: 0.5mg/ml | Purification: Affinity-chromatography
Applications: WB, IHC
Observed MW: 70 kDa | Calculated MW: 27383 MW
Cellular localization: Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .
Tissue details: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .
Status: Active
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