| Field | Specification |
|---|---|
| Target | |
| Alternative names | Polycomb complex protein BMI-1;Polycomb group RING finger protein 4;RING finger protein 51;BMI1;PCGF4, RNF51; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Bmi1, different from the related mouse sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects polycomb complex protein BMI-1 (gene BMI1) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 36.9 kDa, and the supplier reports an observed band at about 40 kDa.
BMI1 is a core component of the Polycomb repressive complex 1 (PRC1), where it stimulates RING1B-mediated monoubiquitination of histone H2A at lysine 119 to keep target genes silent. It represses the INK4A/ARF locus and is required for self-renewal of hematopoietic, neural and other stem cells, and it is studied in stem cell biology and cancer.
| Target | Polycomb complex protein BMI-1 (gene BMI1; UniProt P35226, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 36.9 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human 293T cells, human K562 cells, rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Synthetic peptide from the middle region of human bmi1. It differs from the mouse sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places polycomb complex protein BMI-1 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
