| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 15; BMP-15; Growth/differentiation factor 9B; GDF-9B; BMP15; GDF9B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BMP15, which shares 70% and 66.7% amino acid (aa) sequence identity with mouse and rat BMP15, respectively. |
| Molecular weight | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects bone morphogenetic protein 15 (gene BMP15) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 45.1 kDa, and the supplier reports an observed band at about 45 kDa.
Bone morphogenetic protein 15 (BMP15) is an oocyte-derived growth factor of the TGF-β superfamily. It acts on surrounding granulosa cells, often together with GDF9, to promote their growth and follicle development, and variants in BMP15 are linked to ovarian insufficiency and differences in ovulation rate in several species.
| Target | Bone morphogenetic protein 15 (gene BMP15; UniProt O95972, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 45.1 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human placenta tissue, human Caco-2 cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, human gastric cancer tissue, human intestinal cancer tissue, human lung cancer tissue, human ovary cancer tissue, human placenta tissue, human prostatic cancer tissue, human thyroid cancer tissue, human tonsil tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human BMP15. Sequence identity with the mouse and rat orthologs: 70% and 66.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes bone morphogenetic protein 15 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tong Wu et al. (2022) Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice. Journal of Functional Biomaterials. 10.3390/jfb13040165
