| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 15; BMP-15; Growth/differentiation factor 9B; GDF-9B; BMP15; GDF9B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BMP15 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22B50 is a rabbit monoclonal antibody that recognizes bone morphogenetic protein 15 (gene BMP15) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 45.1 kDa, and the supplier reports an observed band at about 50 kDa.
Bone morphogenetic protein 15 (BMP15) is an oocyte-derived growth factor of the TGF-β superfamily. It acts on surrounding granulosa cells, often together with GDF9, to promote their growth and follicle development, and variants in BMP15 are linked to ovarian insufficiency and differences in ovulation rate in several species.
| Target | Bone morphogenetic protein 15 (gene BMP15; UniProt O95972, human) |
|---|---|
| Clone | 22B50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.1 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BMP15.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes bone morphogenetic protein 15 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
