| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;BMP2;BMP2A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-2, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects bone morphogenetic protein 2 (gene BMP2) in human and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 44.7 kDa; the supplier reports observed bands at 20 kDa, 40 kDa.
Bone morphogenetic protein 2 (BMP2) is a TGF-β superfamily growth factor that binds type I and type II BMP receptors and activates SMAD1/5/8 signaling. It is a strong inducer of bone and cartilage formation and also shapes heart, neural and limb development, which makes it a standard factor in osteogenesis and developmental biology research.
| Target | Bone morphogenetic protein 2 (gene BMP2; UniProt P12643, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 44.7 kDa |
| Observed MW | 20 kDa, 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| ELISA (capture antibody) | 1–5 µg/mL (Human) |
Samples with a confirmed band (WB): rat lung tissue, rat brain tissue, U87 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human BMP-2. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt describes bone morphogenetic protein 2 as a secreted protein. Tissue expression noted by UniProt (human): Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Xiang Yazhou et al. (2022) Microparticles from Hyperphosphatemia-Stimulated Endothelial Cells Promote Vascular Calcification Through Astrocyte-Elevated Gene-1. Calcified Tissue International. 10.1007/s00223-022-00960-6
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