| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 4;BMP-4;Bone morphogenetic protein 2B;BMP-2B;BMP4;BMP2B, DVR4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-4, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects bone morphogenetic protein 4 (gene BMP4) in human and mouse samples and is validated for ELISA and Western blot. The predicted molecular weight is 46.6 kDa, and the supplier reports an observed band at about 46 kDa.
Bone morphogenetic protein 4 (BMP4) is a TGF-β superfamily growth factor that activates SMAD1/5/8 through BMP receptors. It patterns the early embryo, including ventral mesoderm and epidermal fates, and regulates bone, kidney, lung and tooth development, and it is widely used to direct stem cell differentiation in culture.
| Target | Bone morphogenetic protein 4 (gene BMP4; UniProt P12644, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 46.6 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
Samples with a confirmed band (WB): mouse lung tissue, mouse liver tissue, HEPA cells, HepG2 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human BMP-4. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Expressed in the lung and lower levels seen in the kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cui ZS et al. (2015) Local expression and role of BMP-2/4 in injured spinal cord. Genetics and Molecular Research. 10.4238/2015.august.7.20
