| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 6;BMP-6;VG-1-related protein;VG-1-R;VGR-1;BMP6;VGR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BMP6, different from the related mouse sequence by six amino acids, and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes bone morphogenetic protein 6 (gene BMP6) in human samples and is validated for ELISA, flow cytometry and Western blot. The predicted molecular weight is 57.2 kDa, and the supplier reports an observed band at about 43 kDa.
Bone morphogenetic protein 6 (BMP6) is a TGF-β superfamily growth factor that promotes bone and cartilage formation. It is also the main BMP ligand that controls hepcidin expression in the liver in response to iron levels, so it is studied in both skeletal biology and iron homeostasis.
| Target | Bone morphogenetic protein 6 (gene BMP6; UniProt P22004, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 57.2 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Synthetic peptide from the C-terminal region of human BMP6. It differs from the mouse sequence at six residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes bone morphogenetic protein 6 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jun Liao et al. (2023) Naotaifang formula attenuates OGD/R-induced inflammation and ferroptosis by regulating microglial M1/M2 polarization through BMP6/SMADs signaling pathway. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2023.115465
