| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 6; BMP-6; VG-1-related protein; VG-1-R; VGR-1; BMP6; VGR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BMP6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21B45 is a rabbit monoclonal antibody that recognizes bone morphogenetic protein 6 (gene BMP6) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 57.2 kDa, and the supplier reports an observed band at about 42 kDa.
Bone morphogenetic protein 6 (BMP6) is a TGF-β superfamily growth factor that promotes bone and cartilage formation. It is also the main BMP ligand that controls hepcidin expression in the liver in response to iron levels, so it is studied in both skeletal biology and iron homeostasis.
| Target | Bone morphogenetic protein 6 (gene BMP6; UniProt P22004, human) |
|---|---|
| Clone | 21B45 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.2 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells, human HEL cells, human 293T cells, rat PC-12 cells, rat RH35 cells, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human BMP6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes bone morphogenetic protein 6 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiang Feng et al. (2025) BMP6, a potential biomarker of inflammatory fibrosis and promising protective factor for dilated cardiomyopathy. Chinese Medicine. 10.1186/s13020-025-01062-9
Liu Ruimin et al. (2025) 5’tiRNA-35-GlyTCC-3 and 5’tiRNA-33-CysGCA-11 target BMP6, CUL1 and SPR of non-syndromic cleft palate. BMC Oral Health. 10.1186/s12903-025-05661-8
