| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natriuretic peptides B; Brain natriuretic factor prohormone; preproBNP; proBNP; Gamma-brain natriuretic peptide; Iso-ANP; NPPB |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BNP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17N42 is a rabbit monoclonal antibody that recognizes natriuretic peptides B (gene NPPB) in human samples and is validated for Western blot. The predicted molecular weight is 14.7 kDa, and the supplier reports an observed band at about 30 kDa.
Brain natriuretic peptide (BNP), encoded by NPPB, is a cardiac hormone secreted mainly by ventricular muscle when the heart wall is stretched. It acts through NPR1 and cGMP to promote natriuresis, diuresis and vasodilation. BNP and its N-terminal fragment NT-proBNP are widely used biomarkers of heart failure.
| Target | Natriuretic peptides B (gene NPPB; UniProt P16860, human) |
|---|---|
| Clone | 17N42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.7 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BNP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places natriuretic peptides B in the [NT-proBNP]: Secreted. Tissue expression noted by UniProt (human): [Brain natriuretic peptide 32]: Detected in the cardiac atria (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wu Teng et al. (2020) An MRTF-A–Sp1–PDE5 Axis Mediates Angiotensin-II-Induced Cardiomyocyte Hypertrophy. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2020.00839
