| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone sialoprotein 2;Bone sialoprotein II;BSP II;Cell-binding sialoprotein;Integrin-binding sialoprotein;IBSP;BNSP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein, different from the related rat and mouse sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin-binding sialoprotein (gene IBSP) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 35.1 kDa, and the supplier reports an observed band at about 37 kDa.
Integrin-binding sialoprotein (IBSP, also called bone sialoprotein) is a highly glycosylated protein of the SIBLING family found in mineralized tissues such as bone, dentin and calcified cartilage. It binds tightly to hydroxyapatite and supports cell attachment through the αvβ3 integrin, and it is a common marker of osteoblast differentiation and bone formation.
| Target | Integrin-binding sialoprotein (gene IBSP; UniProt P21815, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 35.1 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
Samples with confirmed staining (IHC): human osteosarcoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human bone Sialoprotein. It differs from the rat and mouse sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes integrin-binding sialoprotein as a secreted protein. Tissue expression noted by UniProt (human): Expressed in bone (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Li Qiang et al. (2020) VPS4B mutation impairs the osteogenic differentiation of dental follicle cells derived from a patient with dentin dysplasia type I. International Journal of Oral Science. 10.1038/s41368-020-00088-z
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