| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bactericidal permeability-increasing protein;BPI;CAP 57;BPI; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human BPI |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-BPI Rabbit Monoclonal Antibody is an antibody targeting BPI. Common applications include WB, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FFE-2; isotype: Rabbit IgG; reactivity: Human; observed MW: 33 kDa; calculated MW: 53900 MW.
Boster Bio Anti-BPI Rabbit Monoclonal Antibody catalog # M01444. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: BPI — Bactericidal permeability-increasing protein
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FFE-2; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 33 kDa; Calculated: 53900 MW
Biological background
Protein function (datasheet): The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. .
Cellular localization (datasheet): Secreted . Cytoplasmic granule membrane . Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. .
Tissue details (datasheet): Restricted to cells of the myeloid series.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Biology,Cell Death,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Metabolism Processes,Pathways and Processes,T Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
