| Field | Specification |
|---|---|
| Target | |
| Alternative names | Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase; Golgi-resident PAP phosphatase; gPAPP; 3'(2'), 5'-bisphosphate nucleotidase 2; Inositol monophosphatase domain-containing protein 1; Myo-inositol monophosphatase A3; Phosphoadenosine phosphate 3'-nucleotidase; BPNT2; IMPA3; IMPAD1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BPNT2 recombinant protein (Position: D62-E354). Human BPNT2 shares 93.2% and 93.5% amino acid (aa) sequence identity with mouse and rat BPNT2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase (gene BPNT2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 38.7 kDa, and the supplier reports an observed band at about 42 kDa.
BPNT2 (also called IMPAD1 or gPAPP) is a Golgi-resident phosphatase that hydrolyzes 3′-phosphoadenosine 5′-phosphate (PAP), a byproduct of sulfotransferase reactions, to AMP. It is needed for efficient sulfation of glycosaminoglycans in cartilage, and loss of its function causes skeletal dysplasia with joint dislocations.
| Target | Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase (gene BPNT2; UniProt Q9NX62, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.7 kDa |
| Observed MW | 42 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells, human A549 cells, human U251 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human liver cancer tissue, human colon adenocarcinoma tissue, human meningioma tissue, human ovarian cancer tissue, human tonsil tissue, human urothelial carcinoma tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SiHa cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human BPNT2 fragment (Asp62–Glu354), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.2% and 93.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase in the Golgi apparatus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
