| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-box transcription factor T; Brachyury protein; Protein T; TBXT; T |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Brachyury |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20T93 is a rabbit monoclonal antibody that recognizes T-box transcription factor T (gene TBXT) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 47.4 kDa; the supplier reports observed bands at 47, 41 kDa.
Brachyury (TBXT) is the founding member of the T-box transcription factor family. It binds T-box sites to activate genes needed for mesoderm formation and notochord development, and it is widely used as an early mesoderm marker in stem cell differentiation and as a marker of chordoma.
| Target | T-box transcription factor T (gene TBXT; UniProt O15178, human) |
|---|---|
| Clone | 20T93 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.4 kDa |
| Observed MW | 47, 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:100 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): MUG-Chor1 (human sacral bone chordoma) cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human brachyury.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places T-box transcription factor T in the nucleus. Tissue expression noted by UniProt (human): Detected in testis, but not in other, normal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
