| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bromodomain-containing protein 2; O27.1.1; BRD2; KIAA9001; RING3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BRD2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20B34 is a rabbit monoclonal antibody that recognizes bromodomain-containing protein 2 (gene BRD2) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 88.1 kDa, and the supplier reports an observed band at about 110 kDa.
BRD2 is a member of the BET family of chromatin readers, whose two bromodomains bind acetylated histones. It helps recruit transcriptional regulators to active genes and contributes to chromatin boundary function together with CTCF. BRD2 is studied in transcriptional regulation, inflammation and metabolism, and it is a target of BET inhibitors such as JQ1.
| Target | Bromodomain-containing protein 2 (gene BRD2; UniProt P25440, human) |
|---|---|
| Clone | 20B34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.1 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): NCCIT cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human BRD2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bromodomain-containing protein 2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
