| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription activator BRG1;3.6.4.-;ATP-dependent helicase SMARCA4;BRG1-associated factor 190A;BAF190A;Mitotic growth and transcription activator;Protein BRG-1;Protein brahma homolog 1;SNF2-beta;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4;SMARCA4;BAF190A, BRG1, SNF2B, SNF2L4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BRG1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GFB-19 is a rabbit monoclonal antibody that recognizes SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 (gene SMARCA4) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 184.6 kDa, and the supplier reports an observed band at about 220 kDa.
BRG1 (SMARCA4) is an ATPase subunit of the SWI/SNF (BAF) chromatin remodeling complexes, which use ATP energy to shift nucleosomes and control access to DNA. It regulates many developmental and tissue-specific genes, and is frequently mutated or lost in several cancers, including lung cancer and small cell carcinoma of the ovary, hypercalcemic type.
| Target | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 (gene SMARCA4; UniProt P51532, human) |
|---|---|
| Clone | GFB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 184.6 kDa |
| Observed MW | 220 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human HEL cells, human MCF-7 cells, human PC-3 cells, human SH-SY5Y cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse thymus tissue, mouse RAW264.7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed result (IP): HEL cells.
Immunogen
Synthetic peptide based on the sequence of human BRG1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 in the nucleus. Tissue expression noted by UniProt (human): Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
