| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A; BUB1; BUB1L |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Bub1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19B32 is a rabbit monoclonal antibody that recognizes mitotic checkpoint serine/threonine-protein kinase BUB1 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 122.4 kDa, and the supplier reports an observed band at about 140 kDa.
BUB1 is a mitotic serine/threonine kinase that binds kinetochores and is needed for the spindle assembly checkpoint and correct chromosome alignment. It phosphorylates histone H2A at Thr120, which recruits shugoshin to protect centromeric cohesion and helps localize the chromosomal passenger complex.
| Target | Mitotic checkpoint serine/threonine-protein kinase BUB1 (gene BUB1; UniProt O43683, human) |
|---|---|
| Clone | 19B32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 122.4 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human bub1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitotic checkpoint serine/threonine-protein kinase BUB1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): High expression in testis and thymus, less in colon, spleen, lung and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
